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Development and applications of CRISPR-Cas9 for genome engineering

Hsu PD, Lander ES, Zhang F.  2014.  Development and applications of CRISPR-Cas9 for genome engineering. Cell. 157(6):1262-78. Google Scholar PubMed

Crystal structure of Cas9 in complex with guide RNA and target DNA.

Nishimasu H, F Ran A, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O.  2014.  Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 156(5):935-49. Google Scholar PubMed

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

F Ran A, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y et al..  2013.  Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell. 154(6):1380-9. Google Scholar PubMed

DNA targeting specificity of RNA-guided Cas9 nucleases.

Hsu PD, Scott DA, Weinstein JA, F Ran A, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O et al..  2013.  DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 31(9):827-32. Google Scholar PubMed

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.

Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA.  2013.  Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41(15):7429-37. Google Scholar PubMed

Multiplex genome engineering using CRISPR/Cas systems.

Cong L, F Ran A, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA et al..  2013.  Multiplex genome engineering using CRISPR/Cas systems. Science. 339(6121):819-23. Google Scholar PubMed