Genome engineering using the CRISPR-Cas9 system.
. 2013. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 8(11):2281-308. Google Scholar PubMed
Posts
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.
/0 Comments/in Publications /by syn-adminF Ran A, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y et al.. 2013. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell. 154(6):1380-9. Google Scholar PubMed
DNA targeting specificity of RNA-guided Cas9 nucleases.
/0 Comments/in Publications /by syn-adminHsu PD, Scott DA, Weinstein JA, F Ran A, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O et al.. 2013. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 31(9):827-32. Google Scholar PubMed
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.
/0 Comments/in Publications /by syn-adminWang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R. 2013. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 153(4):910-8. Google Scholar PubMed
RNA-guided editing of bacterial genomes using CRISPR-Cas systems.
/0 Comments/in Publications /by syn-adminJiang W, Bikard D, Cox D, Zhang F, Marraffini LA. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 31(3):233-9. Google Scholar PubMed
Multiplex genome engineering using CRISPR/Cas systems.
/0 Comments/in Publications /by syn-adminCong L, F Ran A, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA et al.. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science. 339(6121):819-23. Google Scholar PubMed
Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers.
/0 Comments/in Publications /by syn-adminBriggs AW, Rios X, Chari R, Yang L, Zhang F, Mali P, Church GM. 2012. Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic Acids Res. 40(15):e117. Google Scholar PubMed
Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription.
/0 Comments/in Publications /by syn-adminZhang F, Cong L, Lodato S, Kosuri S, Church GM, Arlotta P. 2011. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 29(2):149-53. Google Scholar PubMed
Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events.
/0 Comments/in Publications /by syn-adminOliveira PH, Prather KJones, Prazeres DMF, Monteiro GA. 2010. Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events. Appl Microbiol Biotechnol. 87(6):2157-67. Google Scholar PubMed
Engineering a direct and inducible protein-RNA interaction to regulate RNA biology.
/0 Comments/in Publications /by syn-adminBelmont BJ, Niles JC. 2010. Engineering a direct and inducible protein-RNA interaction to regulate RNA biology. ACS Chem Biol. 5(9):851-61. Google Scholar PubMed
