Posts

Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK.  2014.  Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells. Mol Cell. 54(4):698-710. Google Scholar PubMed

Crystal structure of Cas9 in complex with guide RNA and target DNA.

Nishimasu H, F Ran A, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O.  2014.  Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell. 156(5):935-49. Google Scholar PubMed

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

F Ran A, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y et al..  2013.  Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell. 154(6):1380-9. Google Scholar PubMed

DNA targeting specificity of RNA-guided Cas9 nucleases.

Hsu PD, Scott DA, Weinstein JA, F Ran A, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O et al..  2013.  DNA targeting specificity of RNA-guided Cas9 nucleases. Nat Biotechnol. 31(9):827-32. Google Scholar PubMed

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.

Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA.  2013.  Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41(15):7429-37. Google Scholar PubMed

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R.  2013.  One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell. 153(4):910-8. Google Scholar PubMed

RNA-guided editing of bacterial genomes using CRISPR-Cas systems.

Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA.  2013.  RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. 31(3):233-9. Google Scholar PubMed

Multiplex genome engineering using CRISPR/Cas systems.

Cong L, F Ran A, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA et al..  2013.  Multiplex genome engineering using CRISPR/Cas systems. Science. 339(6121):819-23. Google Scholar PubMed

Inducible control of subcellular RNA localization using a synthetic protein-RNA aptamer interaction

Belmont BJ, Niles JC.  2012.  Inducible control of subcellular RNA localization using a synthetic protein-RNA aptamer interaction. PLoS One. 7(10):e46868. Google Scholar PubMed

Engineering a direct and inducible protein-RNA interaction to regulate RNA biology.

Belmont BJ, Niles JC.  2010.  Engineering a direct and inducible protein-RNA interaction to regulate RNA biology. ACS Chem Biol. 5(9):851-61. Google Scholar PubMed